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Hi everyone, for my Biology EE I was thinking of doing an experiment where I test out different temperatures on the heat shock during bacterial transformation. I've decided to test the effect of temperature because the protocol I'm using has very specific temperatures and times when merging the bacteria (E.coli) and plasmid containing the antibacterial resistant gene, so therefore I was curious as to what will happen if I were to change the temperature slightly. Do you guys have any suggestions? What do you think of this as a Biology EE lab?