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Lipase Enzyme

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I've been having trouble finding results for my bio IA. I can't seem to make the lipase react and change colours..... Right now, I only have 1 more hour till I don't get any more class time to work on my IA. I need some help... What can I do?

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My substrate is 2% milk. I'm changing it's concentration.

So first I put the conc into the testtubes then i added phenophalein.

i put into 37degrees water bath then @ 37degrees, i poured enzymes in.... nothing happened. :S

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Did you add buffer solution? Also, did you try using a higher concentration? 2% seems very low to me. Also, why didn't you try using something that has more fat in it like oil or margarine?

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what's a buffer solution?

if i use oil or magarine... i can't dilute it and those factors are already taken by someone else so I can't experiment on them even if I had the choice.

Is there something else I could change :S?

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Get 100% milk and dilute it. The thing is, I am just trying to think of a reason as to why the reaction hasnt shown, and thats the only thing I can think of. I mean 2% is just too low, thats like having 20ml of milk in 1000ml (1l) of water.

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mhm. thank you very much!

so i'm just going to ask for some clarification

with 100% milk, i dilute it into whatever dilutions i want.

i add phenophalien and mix. then i put it in optimal temp (37 degrees)

Then i pour enzyme and wait for it to turn colours.

what if it doesn't turn colours? what do i write on my IA then?

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Okay with dilutions most people do 25,50,75 and 100%, others use 20,40,60, 80 and 100%.

Yes, add few drop of phenolphthalein then in water bath for optimum temperature, then add the enzyme and start the stop clock.

If it doesn't change color then there's something wrong with the lipase you have. If you are going to stick to it, then mention that in your IA and say that there are other factors that may have caused this, for example didn't wait for the water to warm up properly.

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