Jovin Teng Posted October 5, 2013 Report Share Posted October 5, 2013 Hey everyone, I am writing up my biology extended essay based on investigating the effects of changing heat shocking temperatures on the transformation efficiency of E. coli. I have already started writing up my EE (background information, variables, hypotheses, etc.), gotten to a decent number of words, but I haven't come around to actually start doing the experiment and collect data... To put it simply, I am kinda lost as to how many trials I need to have, and also the amount of cells (ul) I would need in total (my supervisor said a transformation uses around 25-50ul cells). My temperatures are going to be 18, 27, 36, 42, 50 degrees Celsius. Please help me out!! I hope to be able to start collecting data this week! Thanks! 1 Reply Link to post Share on other sites More sharing options...
MainRostand Posted October 6, 2013 Report Share Posted October 6, 2013 Well, to make your data more reliable and precise you always need to repeat your experiment at least 3 times and obtain averages. It would almost be a crime to forget to do something like this for your EE! Maybe, because it is really important, you will want to repeat it 5 times. But that's up to you, I would have a standard of 3 times. Remember the experiment is really important in Bio extended essays! 1 Reply Link to post Share on other sites More sharing options...
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